Dual roles of human BubR1, a mitotic checkpoint kinase, in the monitoring of chromosomal instability

• Published in: Cancer cell Vol. 4; no. 6; pp. 483 - 497
• Main Authors: Shin, Hyun-Jin, Baek, Kwan-Hyuck, Jeon, Ae-Hwa, Park, Moon-Taek, Lee, Su-Jae, Kang, Chang-Mo, Lee, Hyun-Sook, Yoo, Seong-Ho, Chung, Doo-Hyun, Sung, Young-Chul, McKeon, Frank, Lee, Chang-Woo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2003
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - physiopathology; Animals; Antineoplastic Agents - pharmacology; Apoptosis - physiology; Caspases - metabolism; Cell Cycle Proteins; Chromosomal Instability - genetics; Cloning, Molecular; Colonic Neoplasms - metabolism; Colonic Neoplasms - physiopathology; hBubR1 protein; HeLa Cells; Humans; Immunohistochemistry; Mice; Mitosis - physiology; Nocodazole - pharmacology; Phosphorylation; Polyploidy; Protein Denaturation; Protein Kinases - metabolism; Protein-Serine-Threonine Kinases; Spindle Apparatus; Transplantation, Heterologous - pathology; Ubiquitin - metabolism

In this study, we show that the formation of polyploidy following sustained mitotic checkpoint activation appears to be preceded by the ubiquitin-dependent proteolysis of hBubR1. In addition, the level of hBubR1 is significantly reduced not only in polyploid cells created by sustained mitotic spindl...