Quantitative targeted absolute proteomics of human blood–brain barrier transporters and receptors

• Published in: Journal of neurochemistry Vol. 117; no. 2; pp. 333 - 345
• Main Authors: Uchida, Yasuo, Ohtsuki, Sumio, Katsukura, Yuki, Ikeda, Chiemi, Suzuki, Takashi, Kamiie, Junichi, Terasaki, Tetsuya
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.04.2011; Wiley-Blackwell
• Subjects: Adolescent; Adult; Age Factors; Aged; Animals; Biological and medical sciences; Biological Transport - physiology; Blood; Blood-Brain Barrier - metabolism; Brain; Brain - cytology; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges; Chromatography, Liquid - methods; Fundamental and applied biological sciences. Psychology; Gynecology. Andrology. Obstetrics; human blood–brain barrier; Humans; liquid chromatography–tandem mass spectrometry; Male; Mammary gland diseases; Medical sciences; Membrane Proteins - metabolism; Membrane Transport Proteins - metabolism; Mice; Middle Aged; Neurons; Proteomics; Proteomics - methods; quantitative targeted absolute proteomics; receptors; Receptors, Cell Surface - metabolism; Rodents; species difference; Tandem Mass Spectrometry - methods; transporters; Tumors; Vertebrates: nervous system and sense organs; Young Adult; Cerebral cortex; Membrane protein; Breast disease; Peptides; transporters; Central nervous system; P Glycoprotein; human blood―brain barrier; Male; Blood brain barrier; Encephalon; quantitative targeted absolute proteomics; Biological receptor; Human; Drug; Multidrug-resistant protein; receptors; Rodentia; Breast cancer; Malignant tumor; Resistance; species difference; Mammary gland diseases; Vertebrata; Organic anion; Membrane receptor; Mammalia; Mouse; liquid chromatography―tandem mass spectrometry; Cancer

J. Neurochem. (2011) 117, 333–345. We have obtained, for the first time, a quantitative protein expression profile of membrane transporters and receptors in human brain microvessels, that is, the blood–brain barrier (BBB). Brain microvessels were isolated from brain cortexes of seven males (16–77 ye...