Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices

• Published in: Environmental microbiology Vol. 9; no. 9; pp. 2364 - 2374
• Main Authors: He, Ji-zheng, Shen, Ju-pei, Zhang, Li-mei, Zhu, Yong-guan, Zheng, Yuan-ming, Xu, Ming-gang, Di, Hongjie
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.09.2007; Blackwell Publishing Ltd
• Subjects: ammonia; Ammonia - metabolism; Archaea; Archaea - classification; Archaea - genetics; Archaea - growth & development; Archaea - metabolism; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - growth & development; Bacteria - metabolism; biogeochemical cycles; China; classification; community structure; Ecosystem; ecosystems; fallow; Fertilizers; Fertilizers - microbiology; field experimentation; genes; genetics; growth & development; metabolism; microbiology; Molecular Sequence Data; nitrification; nitrogen fertilizers; Nitrosospira; nucleotide sequences; nutrients; oxidation; Oxidation-Reduction; phosphorus; Phylogeny; polymerase chain reaction; Population Dynamics; population size; potassium; quantitative analysis; RNA, Ribosomal, 16S; RNA, Ribosomal, 16S - classification; soil bacteria; Soil Microbiology; soil pH; summer; upland soils; winter; China

The abundance and composition of soil ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were investigated by using quantitative real-time polymerase chain reaction, cloning and sequencing approaches based on amoA genes. The soil, classified as agri-udic ferrosols with pH (H₂O) ran...