Studies of the reactivity of artificial peroxidase-like hemoproteins based on antibodies elicited against a specifically designed ortho-carboxy substituted tetraarylporphyrin

• Published in: FEBS letters Vol. 443; no. 2; pp. 229 - 234
• Main Authors: de Lauzon, Solange, Desfosses, Bernard, Mansuy, Daniel, Mahy, Jean-Pierre
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 29.01.1999
• Subjects: Antibodies - metabolism; Artificial hemoprotein; Catalysis; Catalytic antibody; Enzyme Stability; Hemeproteins - metabolism; Hydrogen-Ion Concentration; Kinetics; Oxidation-Reduction; Peroxidase; Peroxidases - metabolism; Porphyrin; Porphyrins - immunology; Substrate Specificity; Catalytic antibody; Artificial hemoprotein; Peroxidase; Porphyrin

The temperature and pH dependence as well as the selectivity of the peroxidase activity of a complex associating a monoclonal antibody 13G10 with its iron(III)-α,α,α,β- meso- tetrakis( ortho-carboxyphenyl) porphyrin (Fe(ToCPP)) hapten have been studied and compared to those of Fe(ToCPP) alone. It fi...