Measurement of basophil-activating capacity of grass pollen allergens, allergoids and hypoallergenic recombinant derivatives by flow cytometry using anti-CD203c

• Published in: Clinical and experimental allergy Vol. 33; no. 9; pp. 1266 - 1272
• Main Authors: Kahlert, H., Cromwell, O., Fiebig, H.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.09.2003; Blackwell; Wiley Subscription Services, Inc
• Subjects: Adult; allergen derivative; Allergens - immunology; allergoid; allergoids; Antibodies, Monoclonal; basophil activation; Basophils - immunology; Biological and medical sciences; CD203c; CD203c antigen; Dose-Response Relationship, Drug; Female; Flow Cytometry - methods; Histamine Release - immunology; Humans; Hypersensitivity - immunology; hypoallergenic allergen variants; Immunoglobulin E - immunology; Immunopathology; Immunotherapy (general aspects); Male; Medical sciences; Middle Aged; Phl p 1; Phl p 5; Phl p 5b antigen; Phl p 6 antigen; Phosphoric Diester Hydrolases - analysis; Plant Extracts - immunology; Poaceae - immunology; Pollen - immunology; Pyrophosphatases - analysis; recombinant allergen; Recombination, Genetic; Human; Immunopathology; Allergy; Monocotyledones; CD203c; hypoallergenic allergen variants; allergen derivative; Activation; Basophil; allergoid; Treatment; Ph1 p 5; recombinant allergen; Gramineae; Immunotherapy; basophil activation; Angiospermae; Ph1 p 1; Spermatophyta; Pollen; Recombinant protein; Molecular biology; Derived product; Allergen
• ISSN: 0954-7894; 1365-2222
• DOI: 10.1046/j.1365-2222.2003.01756.x

Summary Background The assessment of the basophil‐activating potential is an important aspect in the development of improved preparations for specific immunotherapy. The aim of the study was to evaluate the suitability of CD203c expression as a measure of basophil activation to compare allergoids wi...