A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia

• Published in: eLife Vol. 10
• Main Authors: Quidwai, Tooba, Wang, Jiaolong, Hall, Emma A, Petriman, Narcis A, Leng, Weihua, Kiesel, Petra, Wells, Jonathan N, Murphy, Laura C, Keighren, Margaret A, Marsh, Joseph A, Lorentzen, Esben, Pigino, Gaia, Mill, Pleasantine
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 04.11.2021; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: Animals; Cell Biology; Cells; Chlamydomonas reinhardtii - metabolism; Cilia; Cilia - metabolism; ciliary pocket; Coat protein; coatomer; Cytoskeletal Proteins - genetics; Cytoskeletal Proteins - metabolism; Fibroblasts; Gene mutations; Homology; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; intraflagellar transport; Lipids; Localization; membrane cargos; Membrane lipids; Membrane proteins; Membrane vesicles; Membranes; Mice; Microscopy; Mutants; Mutation; Protein Binding; Protein Transport; Proteins; Retrograde transport; Satellites; vesicular traffic; membrane cargos; chlamydomonas reinhardtii; intraflagellar transport; CLEM; coatomer; vesicular traffic; mouse; cell biology; correlative light and electron microscopy; IFT; COPI; cilia; transmission electron microscopy; TEM; ciliary pocket

Intraflagellar transport (IFT) is a highly conserved mechanism for motor-driven transport of cargo within cilia, but how this cargo is selectively transported to cilia is unclear. WDR35/IFT121 is a component of the IFT-A complex best known for its role in ciliary retrograde transport. In the absence...