Gold nanoparticle-adjuvanted S protein induces a strong antigen-specific IgG response against severe acute respiratory syndrome-related coronavirus infection, but fails to induce protective antibodies and limit eosinophilic infiltration in lungs

• Published in: Microbiology and immunology Vol. 64; no. 1; p. 33
• Main Authors: Sekimukai, Hanako, Iwata-Yoshikawa, Naoko, Fukushi, Shuetsu, Tani, Hideki, Kataoka, Michiyo, Suzuki, Tadaki, Hasegawa, Hideki, Niikura, Kenichi, Arai, Katsuhiko, Nagata, Noriyo
• Format: Journal Article
• Language: English
• Published: Australia 01.01.2020
• Subjects: Adjuvants, Immunologic - pharmacology; Analysis of Variance; Animals; Antibodies, Viral - immunology; Chlorocebus aethiops; Coronavirus - immunology; Coronavirus Infections - immunology; Coronavirus Infections - prevention & control; Coronavirus Infections - virology; Cytokines - metabolism; Disease Models, Animal; Female; Gold - chemistry; Immunization; Immunoglobulin G - immunology; Lung - immunology; Lung - pathology; Metal Nanoparticles - chemistry; Mice; Mice, Inbred BALB C; Recombinant Proteins - immunology; Severe Acute Respiratory Syndrome - immunology; Severe Acute Respiratory Syndrome - prevention & control; Severe Acute Respiratory Syndrome - virology; Severe acute respiratory syndrome-related coronavirus - immunology; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - immunology; Toll-Like Receptors; Vaccination; Vaccines, Synthetic; Vero Cells; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Vaccines - immunology; Viral Vaccines - pharmacology; Viral Vaccines - therapeutic use; coronavirus; eosinophils; immunopathology; mouse model; adjuvant; gold nanoparticles

The spike (S) protein of coronavirus, which binds to cellular receptors and mediates membrane fusion for cell entry, is a candidate vaccine target for blocking coronavirus infection. However, some animal studies have suggested that inadequate immunization against severe acute respiratory syndrome co...