Inhibition of neurite extension by overexpression of individual domains of LIM kinase 1

• Published in: Journal of neurochemistry Vol. 78; no. 4; pp. 924 - 927
• Main Authors: Birkenfeld, Jörg, Betz, Heinrich, Roth, Dagmar
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.08.2001; Blackwell
• Subjects: actin; Amides - pharmacology; Animals; Biological and medical sciences; Cell Line; Cell Size; cofilin; Enzyme Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; Genes, Reporter; Humans; Immunoblotting; Immunohistochemistry; Isolated neuron and nerve. Neuroglia; LIM kinase 1; Lim Kinases; Mice; Nerve Growth Factor - pharmacology; neurite outgrowth; Neurites - drug effects; Neurites - metabolism; PC12 cells; Protein Kinases - genetics; Protein Kinases - metabolism; Protein Structure, Tertiary; Pyridines - pharmacology; Rats; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Two-Hybrid System Techniques; Vertebrates: nervous system and sense organs; Cell line; Neurite; Enzyme; Growth; Kinase; Transferases; Gene overexpression; Molecular domain

Lin‐11, Isl‐1 and Mec‐3 (LIM) kinases are serine/threonine kinases that phosphorylate cofilin, an actin depolymerizing protein. LIM kinases have a highly modular structure composed of two N‐terminal LIM domains (LIM 1/2), a PSD‐95, Dlg and ZO‐1 (PDZ) domain and a C‐terminal protein kinase domain. He...