Regulatory effect of daphnetin on the balance of Th17 and Treg cells in the peripheral blood mononuclear cells from patients with unexplained recurrent pregnancy loss

• Published in: Central-European journal of immunology Vol. 45; no. 4; pp. 403 - 408
• Main Authors: Zhang, Zhiqin, Long, Shenggen, Huang, Zhihui, Tan, Jun, Wu, Qiongfang, Huang, Ouping
• Format: Journal Article
• Language: English
• Published: Poland Termedia Publishing House 01.01.2020
• Subjects: Clinical Immunology; Cytokines; daphnetin; Enzyme-linked immunosorbent assay; Flow cytometry; Foxp3 protein; Helper cells; Immunoregulation; Interleukin 2; Interleukin 6; Leukocytes (mononuclear); Lymphocytes T; Peripheral blood mononuclear cells; Polymerase chain reaction; Pregnancy; Stat3 protein; Stat5 protein; th17 cells; Transforming growth factor-b1; treg cells; unexplained recurrent pregnancy loss; daphnetin; peripheral blood mononuclear cells; unexplained recurrent pregnancy loss; Treg cells; Th17 cells
• DOI: 10.5114/ceji.2020.103414
• ISSN: 1426-3912

There has a close relationship between the balance of T helper 17 (Th17) cells and Foxp3+ regulatory T cells (Treg) and unexplained recurrent pregnancy loss (URPL). The present study is to investigate the regulatory effect of daphnetin, which is extracted from Daphne odora Var, on the balance of Th17 and Treg cells in the peripheral blood mononuclear cells (PBMCs) from patients with URPL. PBMCs were isolated from 35 pregnant women with URPL and 35 women with normal early pregnancies, respectively and treated with daphnetin for three days. Flow cytometry was performed to measure the proportions of Th17 and Treg cells. The level of expression of IL-6, TGF-β1 and IL-2 were detected using enzyme-linked immunosorbent assay (ELISA) and the level of expression of FoxP3, RORγt, signal transducers and activators of transcriotion 3 (STAT3) and STAT5 were detected by RT-PCR. The concentrations of Th17-type cytokines IL-2 were significantly decreased in the URPL group after treatment (p < 0.01). Treg-type cytokines such as TGF-β1 and IL-6 were significantly increased after treatment (p < 0.01). At the same time, daphnetin may induce a decrease in the ratio of RORγt to Foxp3 and a Treg cell bias, which would be beneficial for pregnancy maintenance. Futhermore the expression level of STAT3 were higher in the URPL patients whereas STAT5 were lower than those in the control subjects. In conclusion, daphnetin may have regulatory effect on the balance of Th17 and Treg cells in the peripheral blood mononuclear cells from patients with URPL.