In vivo and in vitro toxicity evaluation of liposome-encapsulated sirolimus

• Published in: International journal of retina and vitreous Vol. 5; no. 1; p. 35
• Main Authors: Abud, Murilo Batista, Louzada, Ricardo Noguera, Isaac, David Leonardo Cruvinel, Souza, Leonardo Gomes, Dos Reis, Ricardo Gomes, Lima, Eliana Martins, de Ávila, Marcos Pereira
• Format: Journal Article
• Language: English
• Published: England BioMed Central 24.09.2019; BMC
• Subjects: Antibiotics; ARPE-19; Cell growth; Entrapment; Inflammation; Kinases; Laboratories; LES; Lipids; Original; Particle size; Rapamycin; Sirolimus; Studies; Uveitis; Brazil; United States > US; Rio de Janeiro Brazil; Rapamycin; LES; Sirolimus; ARPE-19; Uveitis

To evaluate the in vivo and in vitro toxicity of a new formulation of liposome-encapsulated sirolimus (LES). In vitro experiments were done using ARPE-19 and HRP cells. An MTT assay was used to determine cell metabolic activity and a TUNEL assay for detecting DNA fragmentation. In vivo experiments w...