Biodiversity of Pneumocystis carinii hominis: typing with different DNA regions

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Published in	Journal of Clinical Microbiology Vol. 35; no. 2; pp. 383 - 387
Main Authors	Latouche, S, Ortona, E, Mazars, E, Margutti, P, Tamburrini, E, Siracusano, A, Guyot, K, Nigou, M, Roux, P
Format	Journal Article
Language	English
Published	Washington, DC American Society for Microbiology 01.02.1997
Subjects	AIDS-Related Opportunistic Infections - microbiology Base Sequence Biological and medical sciences DNA, Fungal - genetics DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Genes, Fungal Genetic Variation Humans Italy Molecular Sequence Data Paris Pneumocystis - classification Pneumocystis - genetics Pneumocystis carinii hominis Pneumonia, Pneumocystis - microbiology Polymerase Chain Reaction Protozoa RNA - genetics RNA, Fungal - genetics RNA, Ribosomal, 5S - genetics rRNA Operon Systematics. Geographical distribution. Morphology. Cytology Thymidylate Synthase - genetics Italy Paris France Human Immunopathology Lung disease Protozoa Pneumonia Protozoal disease Typing Genetic variability Genetic marker Respiratory disease Enzyme Transferases Ribosomal DNA Pneumocystis carinii AIDS Parasitosis Thymidylate synthase Immune deficiency Infection Methyltransferases Viral disease DNA Molecular epidemiology Sporozoa
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ISSN	0095-1137 1098-660X
DOI	10.1128/JCM.35.2.383-387.1997

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Abstract	Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: JCM
AbstractList	The purpose of this study was to identify the most useful gene for the detection of biodiversity of Pneumocystis carinii hominis isolates and to compare samples from French and Italian subjects. We studied 20 bronchoalveolar lavage fluid specimens from 20 human immunodeficiency virus-infected patients (10 French and 10 Italian patients) with Pneumocystis carinii pneumonia by DNA sequencing of the thymidylate synthase (TS), 5S rRNA, large-subunit mitochondrial rRNA (mt LSU rRNA), and internal transcribed spacer (ITS1 and ITS2) genes. Thirteen of the 20 sequenced samples had the prototype TS gene sequence. Fourteen of the 20 samples showed the prototype sequence of the 5S rRNA gene, and 6 had variant sequences of the 5S rRNA gene. The mt LSU rRNA gene was sequenced for 18 of the 20 samples; all sequences were different from the prototype sequence and were classified into four groups. Thirteen of the 20 ITS1 and ITS2 sequences were analyzed, and all the sequences were found to be different from the prototype sequence and were classified into 10 groups. The internal transcribed spacer regions thus appear to be the most discriminatory region of DNA for analysis of the biodiversity of P. carinii hominis isolates.The purpose of this study was to identify the most useful gene for the detection of biodiversity of Pneumocystis carinii hominis isolates and to compare samples from French and Italian subjects. We studied 20 bronchoalveolar lavage fluid specimens from 20 human immunodeficiency virus-infected patients (10 French and 10 Italian patients) with Pneumocystis carinii pneumonia by DNA sequencing of the thymidylate synthase (TS), 5S rRNA, large-subunit mitochondrial rRNA (mt LSU rRNA), and internal transcribed spacer (ITS1 and ITS2) genes. Thirteen of the 20 sequenced samples had the prototype TS gene sequence. Fourteen of the 20 samples showed the prototype sequence of the 5S rRNA gene, and 6 had variant sequences of the 5S rRNA gene. The mt LSU rRNA gene was sequenced for 18 of the 20 samples; all sequences were different from the prototype sequence and were classified into four groups. Thirteen of the 20 ITS1 and ITS2 sequences were analyzed, and all the sequences were found to be different from the prototype sequence and were classified into 10 groups. The internal transcribed spacer regions thus appear to be the most discriminatory region of DNA for analysis of the biodiversity of P. carinii hominis isolates. The purpose of this study was to identify the most useful gene for the detection of biodiversity of Pneumocystis carinii hominis isolates and to compare samples from French and Italian subjects. We studied 20 bronchoalveolar lavage fluid specimens from 20 human immunodeficiency virus-infected patients (10 French and 10 Italian patients) with Pneumocystis carinii pneumonia by DNA sequencing of the thymidylate synthase (TS), 5S rRNA, large-subunit mitochondrial rRNA (mt LSU rRNA), and internal transcribed spacer (ITS1 and ITS2) genes. Thirteen of the 20 sequenced samples had the prototype TS gene sequence. Fourteen of the 20 samples showed the prototype sequence of the 5S rRNA gene, and 6 had variant sequences of the 5S rRNA gene. The mt LSU rRNA gene was sequenced for 18 of the 20 samples; all sequences were different from the prototype sequence and were classified into four groups. Thirteen of the 20 ITS1 and ITS2 sequences were analyzed, and all the sequences were found to be different from the prototype sequence and were classified into 10 groups. The internal transcribed spacer regions thus appear to be the most discriminatory region of DNA for analysis of the biodiversity of P. carinii hominis isolates. Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: JCM
Author	S Latouche K Guyot E Tamburrini E Ortona M Nigou E Mazars P Roux A Siracusano P Margutti
AuthorAffiliation	Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire Saint-Antoine, Paris, France
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Keywords	Human Immunopathology Lung disease Protozoa Pneumonia Protozoal disease Typing Genetic variability Genetic marker Respiratory disease Enzyme Transferases Ribosomal DNA Pneumocystis carinii AIDS Parasitosis Thymidylate synthase Immune deficiency Infection Methyltransferases Viral disease DNA Molecular epidemiology Sporozoa
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SubjectTerms	AIDS-Related Opportunistic Infections - microbiology Base Sequence Biological and medical sciences DNA, Fungal - genetics DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Genes, Fungal Genetic Variation Humans Italy Molecular Sequence Data Paris Pneumocystis - classification Pneumocystis - genetics Pneumocystis carinii hominis Pneumonia, Pneumocystis - microbiology Polymerase Chain Reaction Protozoa RNA - genetics RNA, Fungal - genetics RNA, Ribosomal, 5S - genetics rRNA Operon Systematics. Geographical distribution. Morphology. Cytology Thymidylate Synthase - genetics
Title	Biodiversity of Pneumocystis carinii hominis: typing with different DNA regions
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