The Mammalian Brain rsec6/8 Complex

rsec6 and rsec8 are two components of a 17S complex in mammalian brain that is homologous to the yeast 834 kDa Sec6/8/15 complex which is essential for exocytosis. Purification and partial amino acid sequencing of the mammalian rsec6/8 complex reveals that it is composed of eight novel proteins with...

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Published inNeuron (Cambridge, Mass.) Vol. 17; no. 6; pp. 1209 - 1219
Main Authors Hsu, Shu-Chan, Ting, Anthony E, Hazuka, Christopher D, Davanger, Svend, Kenny, James W, Kee, Yun, Scheller, Richard H
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.1996
Subjects
Animals
Brain - metabolism
Carrier Proteins
Cells, Cultured
Hippocampus - cytology
Hippocampus - metabolism
Mammalia
Membrane Proteins - metabolism
Neurons - metabolism
Peptide Mapping
Precipitin Tests
Proteins - genetics
Proteins - isolation & purification
Proteins - metabolism
Qa-SNARE Proteins
Rats
Subcellular Fractions - metabolism
Tissue Distribution
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Summary:rsec6 and rsec8 are two components of a 17S complex in mammalian brain that is homologous to the yeast 834 kDa Sec6/8/15 complex which is essential for exocytosis. Purification and partial amino acid sequencing of the mammalian rsec6/8 complex reveals that it is composed of eight novel proteins with a combined molecular weight of 743 kDa. The complex is broadly expressed in brain and displays a plasma membrane localization in nerve terminals. Membrane associated rsec6/8 complex coimmunoprecipitates with syntaxin, a plasma membrane protein critical for neurotransmission. These data suggest a role for the mammalian rsec6/8 complex in neurotransmitter release via interactions with the core vesicle docking and fusion apparatus.
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ISSN:0896-6273
1097-4199
DOI:10.1016/S0896-6273(00)80251-2