Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks
Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology re...
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| Published in | Journal of pathology and translational medicine Vol. 46; no. 6; pp. 562 - 568 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Korea (South)
Korean Society of Pathologists, Korean Society for Cytopathology
01.12.2012
The Korean Society of Pathologists and The Korean Society for Cytopathology 대한병리학회 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1738-1843 2383-7837 2092-8920 2383-7845 |
| DOI | 10.4132/KoreanJPathol.2012.46.6.562 |
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| Abstract | Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at low cost.
Ordinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks.
By using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes.
Construction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices. |
|---|---|
| AbstractList | Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at low cost.BACKGROUNDAdvances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at low cost.Ordinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks.METHODSOrdinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks.By using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes.RESULTSBy using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes.Construction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices.CONCLUSIONSConstruction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices. Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at low cost. Ordinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks. By using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes. Construction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices. Background: Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at low cost. Methods: Ordinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks. Results: By using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes. Conclusions: Construction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices. [PUBLICATION ABSTRACT] Background: Advances of tissue microarray (TMA) technology have enabled simultaneous in situ ana lysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at lowcost. Methods: Ordinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks. Results: By using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes. Conclusions: Construction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices. KCI Citation Count: 3 |
| Author | Choi, Suk Jin Kim, Lucia Song, Ju Young Han, Jee Young Kim, Kyu Ho Kim, Joon Mee Chu, Young Chae Park, In Suh Choi, Chang Hwan |
| AuthorAffiliation | Department of Pathology, Inha University Hospital, Inha University College of Medicine, Incheon, Korea |
| AuthorAffiliation_xml | – name: Department of Pathology, Inha University Hospital, Inha University College of Medicine, Incheon, Korea |
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| CitedBy_id | crossref_primary_10_7314_APJCP_2015_16_3_1129 crossref_primary_10_1179_2046023614Y_0000000046 crossref_primary_10_1080_01652176_2015_1030713 crossref_primary_10_3390_medicina57121309 crossref_primary_10_1016_j_prp_2023_154430 crossref_primary_10_4132_KoreanJPathol_2013_47_3_238 crossref_primary_10_1089_bio_2019_0011 crossref_primary_10_1038_jid_2014_277 crossref_primary_10_1080_10520295_2021_1973102 crossref_primary_10_1007_s40944_022_00658_5 crossref_primary_10_4132_KoreanJPathol_2014_48_1_17 crossref_primary_10_5937_smclk4_46109 crossref_primary_10_3390_microarrays3020103 |
| Cites_doi | 10.1007/s00428-008-0622-9 10.5348/ijcri-2010-09-1-RA-1 10.1002/ijc.1385 10.1111/j.1365-2559.2007.02713.x 10.1186/1746-1596-6-117 10.1136/jcp.56.2.144 10.1093/hmg/10.7.657 10.1186/1746-1596-1-14 10.4103/0377-4929.54980 10.1136/jcp.2006.039578 10.1016/j.prp.2006.01.014 10.1097/00129039-200412000-00015 10.1097/01.pai.0000213140.47277.f6 10.4103/0377-4929.85139 10.1002/path.893 10.1309/F2Q38DXN1V1V4GQM 10.1016/j.mpdhp.2009.01.010 |
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| Snippet | Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology... Background: Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived... Background: Advances of tissue microarray (TMA) technology have enabled simultaneous in situ ana lysis of biomarker expression in a large number of archived... |
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| SubjectTerms | Bone marrow Methods Original 병리학 |
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| Title | Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/23323107 https://www.proquest.com/docview/1285166209 https://www.proquest.com/docview/1273578847 https://pubmed.ncbi.nlm.nih.gov/PMC3540334 https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001723205 |
| Volume | 46 |
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| ispartofPNX | Journal of Pathology and Translational Medicine, 2012, 46(6), , pp.562-568 |
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