Hedgehog signaling through GLI1 and GLI2 is required for epithelial–mesenchymal transition in human trophoblasts

• Published in: Biochimica et Biophysica Acta (BBA) - General Subjects Vol. 1850; no. 7; pp. 1438 - 1448
• Main Authors: Tang, Chao, Mei, Liu, Pan, Liyu, Xiong, Wenyi, Zhu, Haibin, Ruan, Hongfeng, Zou, Chaochun, Tang, Lanfang, Iguchi, Takuma, Wu, Ximei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2015; Elsevier BV
• Subjects: Blotting, Western; Cadherins; Cadherins - genetics; Cadherins - metabolism; Cell Line; cell movement; Cells, Cultured; chromatin; Epithelial-Mesenchymal Transition; epithelium; Female; Gene Expression; genes; Hedgehog Proteins; Hedgehog Proteins - metabolism; Hedgehog signaling; HEK293 Cells; Humans; Kruppel-Like Transcription Factors; Kruppel-Like Transcription Factors - genetics; Kruppel-Like Transcription Factors - metabolism; Lentivirus; messenger RNA; Microscopy, Confocal; Models, Biological; phenotype; Placenta; Placenta - cytology; Placenta - metabolism; precipitin tests; Pregnancy; Protein Binding; quantitative polymerase chain reaction; Receptors, G-Protein-Coupled; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; repressor proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; Signal Transduction; small interfering RNA; Smoothened Receptor; tissue repair; Transcription Factors; Transcription Factors - genetics; Transcription Factors - metabolism; Trophoblast; Trophoblasts; Trophoblasts - cytology; Trophoblasts - metabolism; vimentin; Western blotting; Zinc Finger Protein GLI1; Zinc Finger Protein Gli2; Hedgehog signaling; Epithelial–mesenchymal transition; Trophoblast
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2015.04.005

Epithelial to mesenchymal transition (EMT) is critical for human placental development, trophoblastic differentiation, and pregnancy-associated diseases. Here, we investigated the effects of hedgehog (HH) signaling on EMT in human trophoblasts, and further explored the underlying mechanism. Human pr...