Spectral properties of the CP43-deletion mutant of Synechocystis sp. PCC 6803

• Published in: Photosynthesis research Vol. 98; no. 1-3; pp. 303 - 314
• Main Authors: Shimada, Yuichiro, Tsuchiya, Tohru, Akimoto, Seiji, Tomo, Tatsuya, Fukuya, Michitaka, Tanaka, Kazunori, Mimuro, Mamoru
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.10.2008; Springer Netherlands; Springer Nature B.V
• Subjects: Bacteria; Bacterial Proteins - genetics; Biochemistry; Biomedical and Life Sciences; Chlorophyll; Cyanobacteria; Fluorescence; Gene Deletion; Life Sciences; Photosynthesis; Photosynthetic Reaction Center Complex Proteins - genetics; Photosystem II Protein Complex - chemistry; Photosystem II Protein Complex - genetics; Photosystem II Protein Complex - physiology; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Regular Paper; Spectrometry, Fluorescence; Spectrum analysis; Synechocystis; Synechocystis - genetics; Synechocystis - physiology; Fluorescence spectrum; sp. PCC 6803; Photosystem II; CP43-deletion mutant; Cyanobacteria
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/s11120-008-9350-x

Spectral properties, particularly fluorescence spectra and their time-dependent behavior, were investigated for a mutant of the cyanobacterium Synechocystis sp. PCC 6803 lacking the 43 kDa chlorophyll-protein (CP43, PsbC). Lack of CP43 was confirmed by a size shift of the corresponding gene and by Western blotting. The CP43-deletion mutant grown under heterotrophic conditions accumulated a small amount of photosystem (PS) II, but virtually no PS II fluorescence was observed. A 686-nm fluorescence band was clearly observed by phycocyanin excitation, coming from the terminal pigments of phycobilisomes. In contrast, no PS I fluorescence was detected by phycocyanin excitation when accumulation of PS II components was not proved by a fluorescence excitation spectrum, indicating that energy transfer to PS I chlorophyll a was mediated by PS II chlorophyll a. Direct connection of phycobilisomes with PS I was not suggested. Based on these fluorescence properties, the energy flow in the CP43-deletion mutant cells is discussed.