Effects of Feeder Cells on Dopaminergic Differentiation of Human Embryonic Stem Cells

• Published in: Frontiers in cellular neuroscience Vol. 10; p. 291
• Main Authors: Zhao, Zhenqiang, Ma, Yanlin, Chen, Zhibin, Liu, Qian, Li, Qi, Kong, Deyan, Yuan, Kunxiong, Hu, Lan, Wang, Tan, Chen, Xiaowu, Peng, Yanan, Jiang, Weimin, Yu, Yanhong, Liu, Xinfeng
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Research Foundation 20.12.2016; Frontiers Media S.A
• Subjects: Cell culture; Cell differentiation; Dopamine; Dopamine receptors; dopaminergic neuron; Electrophysiological properties; Embryo cells; Embryo fibroblasts; Embryos; Excitability; Fibroblasts; Hospitals; human embryonic stem cells; Human foreskin fibroblasts; Immunocytochemistry; Laboratories; Membrane potential; Morphology; Mouse embryonic fibroblasts; neural differentiation; Neurodegeneration; Neurology; Neurons; Neuroscience; Nuclear receptors; Nurr1 protein; Pluripotency; Scanning electron microscopy; Stem cell transplantation; Stem cells; China; human foreskin fibroblasts; mouse embryonic fibroblasts; dopaminergic neuron; neural differentiation; human embryonic stem cells; electrophysiological properties

Mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs) are used for the culture of human embryonic stem cells (hESCs). MEFs and HFFs differed in their capacity to support the proliferation and pluripotency of hESCs and could affect cardiac differentiation potential of hESCs. The ai...