Probing thermostability of detergent-solubilized CB2 receptor by parallel G protein–activation and ligand-binding assays

• Published in: The Journal of biological chemistry Vol. 295; no. 1; pp. 181 - 190
• Main Authors: Beckner, Ryan L., Zoubak, Lioudmila, Hines, Kirk G., Gawrisch, Klaus, Yeliseev, Alexei A.
• Format: Journal Article
• Language: English
• Published: 11200 Rockville Pike, Suite 302, Rockville, MD 20852-3110, U.S.A Elsevier Inc 03.01.2020; American Society for Biochemistry and Molecular Biology
• Subjects: cannabinoid receptor; cannabinoid receptor CB2; drug screening; Facade–TEG detergent; G protein activation; G protein-coupled receptor (GPCR); ligand-binding protein; Membrane Biology; structural biology; thermostability; ligand-binding protein; cannabinoid receptor CB2; drug screening; thermostability; G protein-coupled receptor (GPCR); G protein activation; structural biology; cannabinoid receptor; Facade–TEG detergent

G protein–coupled receptors (GPCRs) comprise a large class of integral membrane proteins involved in the regulation of a broad spectrum of physiological processes and are a major target for pharmaceutical drug development. Structural studies can help advance the rational design of novel specific pha...