Activation of S6 Kinase Activity in 3T3-L1 Cells by Insulin and Phorbol Ester

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 82; no. 13; pp. 4369 - 4373
• Main Authors: Tabarini, Diane, Heinrich, Julia, Rosen, Ora M.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.07.1985; National Acad Sciences
• Subjects: 3T3 L1 cells; Adipocytes; Adipose Tissue - drug effects; Adipose Tissue - enzymology; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Cell extracts; Cell Line; Centrifugation, Density Gradient; Dose-Response Relationship, Drug; Enzyme Activation; Enzymes; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Histones; Insulin; Insulin - pharmacology; Mice; Phorbol esters; Phorbols; Phorbols - pharmacology; Phospholipids; Phosphorylation; Protein Kinases - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Time Factors; Transferases; Adipocyte; Cell culture; Phosphorylation; Enzyme; Rodentia; Activation; Insulin; Ribosomal protein; Protein hormone; Vertebrata; Regulation(control); Mammalia; Cell line; Mouse; Protein kinase; Animal; Growth factor

Treatment of 3T3-L1 cells with 0.1-1.0 nM insulin results in rapid (5-15 min) activation of a soluble protein kinase that phosphorylates serine residues in ribosomal protein S6. The insulin-stimulated kinase activity is detectable in confluent, nongrowing preadipocytes and adipocytes. In the presenc...