Comparative Analysis of Olive-Derived Phenolic Compounds' Pro-Melanogenesis Effects on B16F10 Cells and Epidermal Human Melanocytes

• Published in: International journal of molecular sciences Vol. 25; no. 8; p. 4479
• Main Authors: Cho, Juhee, Bejaoui, Meriem, Tominaga, Kenichi, Isoda, Hiroko
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2024
• Subjects: Aldehydes - pharmacology; Analysis; Animals; B16F10 cells; Cancer; Care and treatment; Cell cycle; Cell Differentiation - drug effects; Cell growth; Cell Line, Tumor; Cyclopentane Monoterpenes; Cytotoxicity; Disease; DNA damage; Enzymes; Epidermal Cells - drug effects; Epidermal Cells - metabolism; Epidermis - drug effects; Epidermis - metabolism; Free radicals (Chemistry); Gene expression; Genes; glutaraldehyde compound; human epidermal melanocyte; Humans; Iridoid Glucosides - pharmacology; Iridoids - pharmacology; Kinases; Melanins - biosynthesis; Melanins - metabolism; Melanocytes - drug effects; Melanocytes - metabolism; Melanogenesis; Melanoma; Melanoma, Experimental - metabolism; Melanoma, Experimental - pathology; Mice; Olea - chemistry; olive-derived natural compound; Phenols - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Polyphenols; Proteins; RNA; Scientific equipment and supplies industry; Skin; Ultraviolet radiation; United States; Japan; melanogenesis; human epidermal melanocyte; olive-derived natural compound; glutaraldehyde compound; B16F10 cells
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25084479

Olive leaf contains plenty of phenolic compounds, among which oleuropein (OP) is the main component and belongs to the group of secoiridoids. Additionally, phenolic compounds such as oleocanthal (OL) and oleacein (OC), which share a structural similarity with OP and two aldehyde groups, are also present in olive leaves. These compounds have been studied for several health benefits, such as anti-cancer and antioxidant effects. However, their impact on the skin remains unknown. Therefore, this study aims to compare the effects of these three compounds on melanogenesis using B16F10 cells and human epidermal cells. Thousands of gene expressions were measured by global gene expression profiling with B16F10 cells. We found that glutaraldehyde compounds derived from olive leaves have a potential effect on the activation of the melanogenesis pathway and inducing differentiation in B16F10 cells. Accordingly, the pro-melanogenesis effect was investigated by means of melanin quantification, mRNA, and protein expression using human epidermal melanocytes (HEM). This study suggests that secoiridoid and its derivates have an impact on skin protection by promoting melanin production in both human and mouse cell lines.