Long- and Short-Range Electrostatic Interactions Affect the Rheology of Highly Concentrated Antibody Solutions

• Published in: Pharmaceutical research Vol. 26; no. 12; pp. 2607 - 2618
• Main Authors: Chari, Ravi, Jerath, Kavita, Badkar, Advait V, Kalonia, Devendra S
• Format: Journal Article
• Language: English
• Published: Boston Boston : Springer US 01.12.2009; Springer US; Springer; Springer Nature B.V
• Subjects: Antibodies - chemistry; Antibodies - metabolism; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Circular Dichroism; Computer based modeling; Computer Simulation; General pharmacology; Hydrogen-Ion Concentration; Medical Law; Medical sciences; Models, Biological; Monoclonal antibodies; Pharmaceutical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Pharmacology/Toxicology; Pharmacy; Proteins; Proteins - metabolism; Research Paper; Rheology; Solutions - chemistry; Static Electricity; Viscosity; computer modeling; viscosity; dipole; highly concentrated protein solutions; protein-protein interactions; Viscosity; Pharmaceutical technology; Antibody; Rheology; Electrostatic interaction; Modeling; Protein; Solution

Purpose To explain the differences in protein-protein interactions (PPI) of concentrated versus dilute formulations of a model antibody. Methods High frequency rheological measurements from pH 3.0 to 12.0 quantitated viscoelasticity and PPI at high concentrations. Dynamic light scattering (DLS) char...