Mitochondrial DNA and Inflammation Are Associated with Cerebral Vessel Remodeling and Early Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

• Published in: Biomolecules (Basel, Switzerland) Vol. 14; no. 4; p. 499
• Main Authors: Petrica, Ligia, Gadalean, Florica, Muntean, Danina Mirela, Jianu, Dragos Catalin, Vlad, Daliborca, Dumitrascu, Victor, Bob, Flaviu, Milas, Oana, Suteanu-Simulescu, Anca, Glavan, Mihaela, Ursoniu, Sorin, Balint, Lavinia, Mogos-Stefan, Maria, Ienciu, Silvia, Cretu, Octavian Marius, Popescu, Roxana, Gluhovschi, Cristina, Iancu, Lavinia, Vlad, Adrian
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2024
• Subjects: Aged; Arteriosclerosis; Asymptomatic; Atherosclerosis; Biomarkers; Carotid artery; Case-Control Studies; Cell adhesion; cerebral vessels; Cerebrovascular disease; Cerebrovascular diseases; Complications and side effects; Creatinine; Cytochrome b; Diabetes; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - genetics; diabetic kidney disease; Diabetic nephropathies; Diabetic Nephropathies - genetics; Diabetic Nephropathies - pathology; DNA, Mitochondrial - genetics; Doppler effect; Female; Flow velocity; Genetic aspects; Genetic testing; Glucosaminidase; Health aspects; Hemodynamics; Humans; Inflammation; Inflammation - genetics; Intercellular adhesion molecule 1; Interleukin 10; Interleukin 18; Kidney diseases; Laboratories; Male; Middle Aged; Mitochondrial DNA; NADH dehydrogenase; Peripheral blood; Tumor necrosis factor-α; Type 2 diabetes; Urine; Vascular Remodeling - genetics; Veins & arteries; Romania; United States > US; diabetic kidney disease; inflammation; mitochondrial DNA; cerebral vessels
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom14040499

Cerebrovascular disease accounts for major neurologic disabilities in patients with type 2 diabetes mellitus (DM). A potential association of mitochondrial DNA ( ) and inflammation with cerebral vessel remodeling in patients with type 2 DM was evaluated. A cohort of 150 patients and 30 healthy controls were assessed concerning urinary albumin/creatinine ratio (UACR), synaptopodin, podocalyxin, kidney injury molecule-1 (KIM-1), N-acetyl-β-(D)-glucosaminidase (NAG), interleukins IL-17A, IL-18, IL-10, tumor necrosis factor-alpha (TNFα), intercellular adhesion molecule-1 (ICAM-1). and nuclear DNA ( ) were quantified in peripheral blood and urine by qRT-PCR. Cytochrome b ( ) gene, subunit 2 of NADH dehydrogenase ( ), and beta 2 microglobulin nuclear gene ( ) were assessed by TaqMan assays. was defined as the ratio of the number of copies, through analysis of the and ratio; cerebral Doppler ultrasound: intima-media thickness (IMT)-the common carotid arteries (CCAs), the pulsatility index (PI) and resistivity index (RI)- the internal carotid arteries (ICAs) and middle cerebral arteries (MCAs), the breath-holding index (BHI). The results showed direct correlations of CCAs-IMT, PI-ICAs, PI-MCAs, RI-ICAs, RI-MCAs with urinary , IL-17A, IL-18, TNFα, ICAM-1, UACR, synaptopodin, podocalyxin, KIM-1, NAG, and indirect correlations with serum , IL-10. BHI correlated directly with serum IL-10, and serum , and negatively with serum IL-17A, serum ICAM-1, and NAG. In neurologically asymptomatic patients with type 2 DM cerebrovascular remodeling and impaired cerebrovascular reactivity may be associated with variations and inflammation from the early stages of diabetic kidney disease.