Novel high-throughput assay to assess cellular manganese levels in a striatal cell line model of Huntington's disease confirms a deficit in manganese accumulation

• Published in: Neurotoxicology (Park Forest South) Vol. 32; no. 5; pp. 630 - 639
• Main Authors: Kwakye, Gunnar F., Li, Daphne, Bowman, Aaron B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2011
• Subjects: Animals; Cell Line; Corpus Striatum - metabolism; Corpus Striatum - pathology; Fura-2; Fura-2 - metabolism; High-throughput assay; High-Throughput Screening Assays - methods; Huntington Disease - genetics; Huntington Disease - metabolism; Huntington's disease; Manganese; Manganese - deficiency; Manganese - metabolism; Metal transport; Mice; Mice, Mutant Strains; Mutation - genetics; Metal transport; Fura-2; Huntington's disease; Manganese; High-throughput assay

In spite of the essentiality of manganese (Mn) as a trace element necessary for a variety of physiological processes, Mn in excess accumulates in the brain and has been associated with dysfunction and degeneration of the basal ganglia. Despite the high sensitivity, limited chemical interference, and...