Novel biosensor system model based on fluorescence quenching by a fluorescent streptavidin and carbazole-labeled biotin

• Published in: Journal of molecular recognition Vol. 29; no. 10; pp. 485 - 491
• Main Authors: Zhu, Xianwei, Shinohara, Hiroaki, Miyatake, Ryuta, Hohsaka, Takahiro
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.10.2016; Wiley Subscription Services, Inc
• Subjects: Biosensing Techniques; Biosensors; Biotin; Biotin - chemistry; Biotin - metabolism; carbazole-labeled biotin; Carbazoles; Carbazoles - chemistry; Carbazoles - metabolism; Couples; Fluorescence; fluorescence quenching; fluorescence recovery; Fluorescent Dyes - chemistry; fluorescent unnatural mutant streptavidin; four-base codon method; Mathematical models; Models, Molecular; molecular biosensor; Mutation; Protein Conformation; Quenching; Quenching (cooling); Spectrometry, Fluorescence; Streptavidin - chemistry; Streptavidin - genetics; Streptavidin - metabolism; Tryptophan - chemistry; Tryptophan - genetics; Tryptophan - metabolism; four-base codon method; molecular biosensor; fluorescence recovery; carbazole-labeled biotin; fluorescent unnatural mutant streptavidin; fluorescence quenching

In the present study, a novel molecular biosensor system model was designed by using a couple of the fluorescent unnatural mutant streptavidin and the carbazole‐labeled biotin. BODIPY‐FL‐aminophenylalanine (BFLAF), a fluorescent unnatural amino acid was position‐specifically incorporated into Trp120...