Metabolic analysis of antibody producing CHO cells in fed-batch production

• Published in: Biotechnology and bioengineering Vol. 110; no. 6; pp. 1735 - 1747
• Main Authors: Dean, Jason, Reddy, Pranhitha
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.2013; Wiley Subscription Services, Inc
• Subjects: Amino Acids - metabolism; Animals; Antibodies, Monoclonal - analysis; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - chemistry; Cell Culture Techniques - methods; Cells; Chinese hamster ovary cells; CHO Cells; Cricetinae; Cricetulus; fed-batch mAb production; Genotype & phenotype; Glucose - metabolism; Industrial production; Isotope Labeling; Isotopes; Lactic Acid - metabolism; metabolic flux analysis; Metabolism; Metabolites; Metabolome; Phosphoenolpyruvate Carboxykinase (ATP) - metabolism; Proteins; Pyruvic Acid - metabolism; Recombinant Proteins - analysis; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Ribose - metabolism; Rodents; stable isotope tracers; Stable isotopes

Chinese hamster ovary (CHO) cells are commonly used for industrial production of recombinant proteins in fed batch or alternative production systems. Cells progress through multiple metabolic stages during fed‐batch antibody (mAb) production, including an exponential growth phase accompanied by lact...