Apparent rate constant mapping using hyperpolarized [1-13C]pyruvate

• Published in: NMR in biomedicine Vol. 27; no. 10; pp. 1256 - 1265
• Main Authors: Khegai, O., Schulte, R. F., Janich, M. A., Menzel, M. I., Farrell, E., Otto, A. M., Ardenkjaer-Larsen, J. H., Glaser, S. J., Haase, A., Schwaiger, M., Wiesinger, F.
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.10.2014; Wiley Subscription Services, Inc
• Subjects: [1-13C]pyruvate; Algorithms; Animals; Carbon-13 Magnetic Resonance Spectroscopy - methods; dynamic metabolic imaging; Female; Humans; hyperpolarized 13C; kinetic modeling; Kinetics; L-Lactate Dehydrogenase - metabolism; Least-Squares Analysis; Mammary Neoplasms, Experimental - chemistry; MCF-7 Cells - chemistry; metabolic exchange rate; Models, Chemical; MRSI; Pyruvates - analysis; Rats, Inbred F344; Signal-To-Noise Ratio; Spheroids, Cellular; Suspensions; Time Factors; hyperpolarized 13C; dynamic metabolic imaging; [1-13C]pyruvate; MRSI; kinetic modeling; metabolic exchange rate

Hyperpolarization of [1‐13C]pyruvate in solution allows real‐time measurement of uptake and metabolism using MR spectroscopic methods. After injection and perfusion, pyruvate is taken up by the cells and enzymatically metabolized into downstream metabolites such as lactate, alanine, and bicarbonate....