Genome-wide DNA methylation analysis of formalin-fixed paraffin embedded colorectal cancer tissue

• Published in: Genes chromosomes & cancer Vol. 53; no. 7; pp. 537 - 548
• Main Authors: Dumenil, Troy D., Wockner, Leesa F., Bettington, Mark, McKeone, Diane M., Klein, Kerenaftali, Bowdler, Lisa M., Montgomery, Grant W., Leggett, Barbara A., Whitehall, Vicki L.J.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.07.2014; Wiley Subscription Services, Inc
• Subjects: Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; CpG Islands; DNA Damage; DNA Methylation; Fixatives; Formaldehyde; Genome, Human; Humans; Paraffin Embedding; Tissue Fixation - methods

Formalin fixation and embedding of clinical tissue samples in paraffin is a common method for archiving biological material. These samples are often well annotated and provide an invaluable resource for research. However, this process of fixation and storage of tissue leads to DNA damage and fragmen...