A technique for relative quantitation of cancer biomarkers in formalin-fixed, paraffin-embedded (FFPE) tissue using stable-isotope-label based mass spectrometry imaging (SILMSI)

• Published in: Journal of mass spectrometry. Vol. 50; no. 9; pp. 1088 - 1095
• Main Authors: Wang, Hong, DeGnore, Jon P., Kelly, Brian D., True, Jan, Garsha, Karl, Bieniarz, Christopher
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.09.2015; Wiley Subscription Services, Inc
• Subjects: Assessments; biomarker; Biomarkers; Cancer; chromogen; formalin-fixed paraffin-embedded (FFPE) tissue; Heterogeneity; imaging mass spectrometry; immunohistochemistry; Ionization; Mass spectrometry; pathology; Receptors; stable isotope labels; imaging mass spectrometry; pathology; stable isotope labels; chromogen; formalin-fixed paraffin-embedded (FFPE) tissue; cancer; immunohistochemistry; biomarker

We developed a novel technique for the relative quantitation of pairs of cancer biomarkers in formalin‐fixed paraffin‐embedded (FFPE) tissue. The method utilizes stable isotope labeled (SIL) chromogens deposited during the standard immunohistochemistry (IHC) tissue staining process. The labeled chro...