Evaluation of the Pig-a, micronucleus, and comet assay endpoints in a 28-day study with ethyl methanesulfonate

• Published in: Environmental and molecular mutagenesis Vol. 55; no. 6; pp. 492 - 499
• Main Authors: Gunther, William C., Coffing, Stephanie L., Dickinson, Donna A., Engel, Maria E., Fiedler, Ronald D., O'Lone, Susan D., Sanok, Kelley E., Thiffeault, Catherine J., Shutsky, Thomas J., Schuler, Maik J., Dobo, Krista L.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.07.2014; Wiley Subscription Services, Inc
• Subjects: Animals; Bioassays; Colon - cytology; Colon - drug effects; Comet Assay - methods; DNA Damage - drug effects; Dose-Response Relationship, Drug; Endpoint Determination; Erythrocytes - drug effects; Ethyl Methanesulfonate - toxicity; Genotoxicity; in vivo genotoxicity; in vivo mutation assay; integration; Liver - cytology; Liver - drug effects; Male; Membrane Proteins - drug effects; Membrane Proteins - genetics; Micronucleus Tests - methods; Mutation Rate; Rats; Rats, Sprague-Dawley; Reticulocytes - drug effects; Swine; integration; in vivo mutation assay; in vivo genotoxicity

Ethyl methanesulfonate (EMS) was evaluated as part of the validation effort for the rat Pig‐a mutation assay and compared with other well‐established in vivo genotoxicity endpoints. Male Sprague‐Dawley (SD) rats were given a daily dose of 0, 6.25, 12.5, 25, 50, or 100 mg/kg/day EMS for 28 days, and...