Immobilization induces anabolic resistance in human myofibrillar protein synthesis with low and high dose amino acid infusion

• Published in: The Journal of physiology Vol. 586; no. 24; pp. 6049 - 6061
• Main Authors: Glover, Elisa I., Phillips, Stuart M., Oates, Bryan R., Tang, Jason E., Tarnopolsky, Mark A., Selby, Anna, Smith, Kenneth, Rennie, Michael J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK The Physiological Society 15.12.2008; Blackwell Publishing Ltd; Blackwell Science Inc
• Subjects: Adult; Amino Acids - administration & dosage; Amino Acids - metabolism; Amino Acids - pharmacology; Amino Acids, Essential - blood; Amino Acids, Essential - metabolism; Dose-Response Relationship, Drug; Elongation Factor 2 Kinase - metabolism; Female; Focal Adhesion Protein-Tyrosine Kinases - metabolism; Glycogen Synthase Kinase 3 - metabolism; Glycogen Synthase Kinase 3 beta; Humans; Immobilization - methods; Infusions, Intravenous; Insulin - blood; Male; Muscle Proteins - biosynthesis; Muscle Strength - drug effects; Muscle Strength - physiology; Myofibrils - drug effects; Myofibrils - metabolism; Myofibrils - physiology; Phosphorylation - drug effects; Protein Kinases - metabolism; Proto-Oncogene Proteins c-akt - metabolism; Quadriceps Muscle - drug effects; Quadriceps Muscle - metabolism; Quadriceps Muscle - physiology; Ribosomal Protein S6 Kinases, 70-kDa - metabolism; Skeletal Muscle and Exercise; TOR Serine-Threonine Kinases; Ubiquitination - drug effects; Young Adult

We tested the hypothesis that increasing blood amino acid (AA) availability would counter the physical inactivity-induced reduction in muscle protein synthesis. We determined how 14 days of unilateral knee immobilization affected quadriceps myofibrillar protein synthesis (MPS) in young healthy subje...