Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia

• Published in: Cell Vol. 184; no. 12; pp. 3192 - 3204.e16
• Main Authors: Asarnow, Daniel, Wang, Bei, Lee, Wen-Hsin, Hu, Yuanyu, Huang, Ching-Wen, Faust, Bryan, Ng, Patricia Miang Lon, Ngoh, Eve Zi Xian, Bohn, Markus, Bulkley, David, Pizzorno, Andrés, Ary, Beatrice, Tan, Hwee Ching, Lee, Chia Yin, Minhat, Rabiatul Adawiyah, Terrier, Olivier, Soh, Mun Kuen, Teo, Frannie Jiuyi, Yeap, Yvonne Yee Chin, Seah, Shirley Gek Kheng, Chan, Conrad En Zuo, Connelly, Emily, Young, Nicholas J., Maurer-Stroh, Sebastian, Renia, Laurent, Hanson, Brendon John, Rosa-Calatrava, Manuel, Manglik, Aashish, Cheng, Yifan, Craik, Charles S., Wang, Cheng-I
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.06.2021; MDPI
• Subjects: Angiotensin-Converting Enzyme 2 - chemistry; Angiotensin-Converting Enzyme 2 - genetics; Angiotensin-Converting Enzyme 2 - immunology; Angiotensin-Converting Enzyme 2 - metabolism; Animals; Antibodies, Neutralizing - chemistry; Antibodies, Neutralizing - immunology; Antibodies, Neutralizing - metabolism; Antigen-Antibody Complex - chemistry; Antigen-Antibody Complex - metabolism; Binding Sites; CHO Cells; coronavirus; COVID-19; COVID-19 - pathology; COVID-19 - virology; Cricetinae; Cricetulus; Cryoelectron Microscopy; Giant Cells - cytology; Giant Cells - metabolism; Humans; Immunology; Life Sciences; Membrane Fusion; Microbiology and Parasitology; Peptide Library; phage display; Protein Binding; Protein Domains; Protein Structure, Quaternary; receptor binding domain (RBD); recombinant monoclonal antibody; SARS-CoV; SARS-CoV-2; SARS-CoV-2 - isolation & purification; SARS-CoV-2 - metabolism; Spike Glycoprotein, Coronavirus - chemistry; Spike Glycoprotein, Coronavirus - immunology; Spike Glycoprotein, Coronavirus - metabolism; Spike protein; syncytia; Virology; COVID-19; SARS-CoV-2; recombinant monoclonal antibody; Spike protein; syncytia; phage display; SARS-CoV; coronavirus; receptor binding domain (RBD)

Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is initiated by binding of the viral Spike protein to host receptor angiotensin-converting enzyme 2 (ACE2), followed by fusion of viral and host membranes. Although antibodies that block this interaction are in emergency use...