Extraction and Genotyping of Cryptosporidium parvum DNA from Fecal Smears on Glass Slides Stained Conventionally for Direct Microscope Examination

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Published inJournal of Clinical Microbiology Vol. 39; no. 1; pp. 401 - 403
Main Authors AMAR, C, PEDRAZA-DIAZ, S, MCLAUCHLIN, J
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.01.2001
SeriesNote
Subjects
Animals
Biological and medical sciences
Cryptosporidiosis - diagnosis
Cryptosporidiosis - parasitology
Cryptosporidium parvum
Cryptosporidium parvum - classification
Cryptosporidium parvum - genetics
DNA, Protozoan - analysis
DNA, Protozoan - genetics
DNA, Protozoan - isolation & purification
Feces - parasitology
Fundamental and applied biological sciences. Psychology
General aspects and techniques
Genotype
Humans
Parasitology
Parasitology - methods
Polymerase Chain Reaction - methods
Protozoa
Staining and Labeling - methods
Polymerase chain reaction
Protozoa
Apicomplexa
Restriction fragment length polymorphism
Typing
Cryptosporidium parvum
DNA
Smear
Genotype
Extraction
Feces
Strain specificity
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Abstract Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
AbstractList A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.
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A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.
ABSTRACT A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.
Author C. Amar
J. McLauchlin
S. Pedraza-Dı́az
AuthorAffiliation Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT, 1 and School of Chemical and Life Sciences, University of Greenwich, Woolwich, London, SE18 8PF, 2 United Kingdom
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10.1016/S0378-1097(97)00115-8
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Keywords Polymerase chain reaction
Protozoa
Apicomplexa
Restriction fragment length polymorphism
Typing
Cryptosporidium parvum
DNA
Smear
Genotype
Extraction
Feces
Strain specificity
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Corresponding author. Mailing address: Food Safety Microbiology Laboratory, Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, 61 Colindale Ave., London, NW9 5HT, United Kingdom. Phone: 44 20 8200 4400, ext. 3505. Fax: 44 20 8200 8264. E-mail: jmclauchlin@phls.nhs.uk.
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A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum...
ABSTRACT A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of...
A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum...
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SubjectTerms Animals
Biological and medical sciences
Cryptosporidiosis - diagnosis
Cryptosporidiosis - parasitology
Cryptosporidium parvum
Cryptosporidium parvum - classification
Cryptosporidium parvum - genetics
DNA, Protozoan - analysis
DNA, Protozoan - genetics
DNA, Protozoan - isolation & purification
Feces - parasitology
Fundamental and applied biological sciences. Psychology
General aspects and techniques
Genotype
Humans
Parasitology
Parasitology - methods
Polymerase Chain Reaction - methods
Protozoa
Staining and Labeling - methods
Title Extraction and Genotyping of Cryptosporidium parvum DNA from Fecal Smears on Glass Slides Stained Conventionally for Direct Microscope Examination
URI http://jcm.asm.org/content/39/1/401.abstract
https://www.ncbi.nlm.nih.gov/pubmed/11136813
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https://pubmed.ncbi.nlm.nih.gov/PMC87744
Volume 39
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