Cystatin D Locates in the Nucleus at Sites of Active Transcription and Modulates Gene and Protein Expression

• Published in: The Journal of biological chemistry Vol. 290; no. 44; pp. 26533 - 26548
• Main Authors: Ferrer-Mayorga, Gemma, Alvarez-Díaz, Silvia, Valle, Noelia, De Las Rivas, Javier, Mendes, Marta, Barderas, Rodrigo, Canals, Francesc, Tapia, Olga, Casal, J. Ignacio, Lafarga, Miguel, Muñoz, Alberto
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.10.2015; American Society for Biochemistry and Molecular Biology
• Subjects: Cell Biology; Cell Line, Tumor; colon cancer; Colonic Neoplasms - genetics; Colonic Neoplasms - metabolism; Colonic Neoplasms - pathology; Core Binding Factor Alpha 1 Subunit - genetics; Core Binding Factor Alpha 1 Subunit - metabolism; Core Binding Factor Alpha 2 Subunit - genetics; Core Binding Factor Alpha 2 Subunit - metabolism; Cystatins - genetics; Cystatins - metabolism; cysteine protease; cytokine; Cytokines - biosynthesis; Cytokines - genetics; gene expression; Gene Expression Regulation, Neoplastic; Humans; MEF2 Transcription Factors - genetics; MEF2 Transcription Factors - metabolism; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; protease inhibitor; Proteomics; Transcription, Genetic; cysteine protease; cytokine; protease inhibitor; colon cancer; gene expression

Cystatin D is an inhibitor of lysosomal and secreted cysteine proteases. Strikingly, cystatin D has been found to inhibit proliferation, migration, and invasion of colon carcinoma cells indicating tumor suppressor activity that is unrelated to protease inhibition. Here, we demonstrate that a proport...