Enhanced transduction efficiency of fiber-substituted adenovirus vectors by the incorporation of RGD peptides in two distinct regions of the adenovirus serotype 35 fiber knob

• Published in: Virus research Vol. 155; no. 1; pp. 48 - 54
• Main Authors: Matsui, Hayato, Sakurai, Fuminori, Katayama, Kazufumi, Kurachi, Shinnosuke, Tashiro, Katsuhisa, Sugio, Kumiko, Kawabata, Kenji, Mizuguchi, Hiroyuki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 2011
• Subjects: Adenoviridae - genetics; Adenovirus; Adenovirus vector; amino acid motifs; Arg-Gly-Asn (RGD) peptide; arginine; asparagine; Capsid Proteins - chemistry; Capsid Proteins - metabolism; CD46; Cell Line; Fiber-mutant; genes; genetic transduction; Genetic Vectors; glycine (amino acid); Humans; innate immunity; integrins; intravenous injection; liver; luciferase; Membrane Cofactor Protein - deficiency; mutants; Oligopeptides - chemistry; Oligopeptides - metabolism; peptides; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Recombination, Genetic; serotypes; Transduction, Genetic; Viral Tropism; viruses; α v-Integrin; α v-Integrin; Arg-Gly-Asn (RGD) peptide; CD46; Adenovirus vector; Fiber-mutant

Fiber-substituted Ad serotype 5 vectors containing the fiber protein from Ad serotype 35 (Ad5F35) exhibit properties that render them suitable as a platform for targeted Ad vectors. Ad5F35 vectors do not show apparent tropism in certain organs, including the liver, and they elicit less innate immuni...