A P450 fusion library of heme domains from Rhodococcus jostii RHA1 and its evaluation for the biotransformation of drug molecules

• Published in: Bioorganic & medicinal chemistry Vol. 23; no. 17; pp. 5603 - 5609
• Main Authors: Kulig, Justyna K., Spandolf, Claudia, Hyde, Ralph, Ruzzini, Antonio C., Eltis, Lindsay D., Grönberg, Gunnar, Hayes, Martin A., Grogan, Gideon
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2015
• Subjects: Biocatalysis; Biotransformation; Cytochrome P-450 Enzyme System - metabolism; Diltiazem; Drug metabolism; Gene Library; Heme - metabolism; Imipramine; Molecular Structure; N-demethylation; P450; P450Rhf; Rhodococcus - metabolism; P450Rhf; Imipramine; Biotransformation; Drug metabolism; P450; N-demethylation; Diltiazem

[Display omitted] The actinomycete Rhodococcus jostii RHA1 contains a multitude of oxygenase enzymes, consonant with its remarkable activities in the catabolism of hydrophobic xenobiotic compounds. In the interests of identifying activities for the transformation of drug molecules, we have cloned ge...