Typing of anastomosis groups of Rhizoctonia solani by restriction analysis of ribosomal DNA

• Published in: Canadian journal of microbiology Vol. 49; no. 9; pp. 556 - 568
• Main Authors: Guillemaut, C, Edel-Hermann, V, Camporota, P, Alabouvette, C, Richard-Molard, M, Steinberg, C
• Format: Journal Article
• Language: English
• Published: Ottawa, Canada NRC Research Press 01.09.2003; National Research Council of Canada; Canadian Science Publishing NRC Research Press
• Subjects: Biological and medical sciences; Crop diseases; Disease; DNA, Fungal - analysis; DNA, Fungal - genetics; DNA, Ribosomal - analysis; DNA, Ribosomal - genetics; Enzymes; Flowers & plants; Fundamental and applied biological sciences. Psychology; Fungi; Genetics; internal transcribed spacers; Life Sciences; Microbiology; Microbiology and Parasitology; Miscellaneous; molecular sequence data; Mycological Typing Techniques; Mycology; nucleotide sequences; Plant diseases; plant pathogenic fungi; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; restriction fragment length polymorphism; Rhizoctonia - classification; Rhizoctonia - genetics; Rhizoctonia solani; Ribonucleic acid; ribosomal DNA; Ribotyping; RNA; Roots (Botany); strain differences; Sugar; Sugar beets; Thanatephorus cucumeris; France; Fungi; Restriction fragment length polymorphism; Typing; Plant pathogen; Ribosomal DNA; Rhizoctonia solani; Identification; Fungi Imperfecti; Thallophyta

A method based on restriction analysis of polymerase chain reaction (PCR)-amplified ribosomal DNA was developed for the rapid characterization of large populations of Rhizoctonia solani at the anastomosis group (AG) level. The restriction maps of the internal transcribed spacers (ITS) sequences were...