Rif1 controls DNA replication by directing Protein Phosphatase 1 to reverse Cdc7-mediated phosphorylation of the MCM complex

• Published in: Genes & development Vol. 28; no. 4; pp. 372 - 383
• Main Authors: Hiraga, Shin-Ichiro, Alvino, Gina M, Chang, Fujung, Lian, Hui-Yong, Sridhar, Akila, Kubota, Takashi, Brewer, Bonita J, Weinreich, Michael, Raghuraman, M K, Donaldson, Anne D
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 15.02.2014
• Subjects: Cell Cycle Proteins - metabolism; DNA Replication - genetics; DNA Replication - physiology; Minichromosome Maintenance Proteins - metabolism; Mutation; Phosphorylation; Protein Phosphatase 1 - metabolism; Protein Serine-Threonine Kinases - metabolism; Protein Structure, Tertiary; Repressor Proteins - genetics; Repressor Proteins - metabolism; Research Paper; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Schizosaccharomyces pombe; Telomere-Binding Proteins - genetics; Telomere-Binding Proteins - metabolism; Temperature; PP1; DNA replication; protein phosphorylation; protein kinase; protein phosphatase

Initiation of eukaryotic DNA replication requires phosphorylation of the MCM complex by Dbf4-dependent kinase (DDK), composed of Cdc7 kinase and its activator, Dbf4. We report here that budding yeast Rif1 (Rap1-interacting factor 1) controls DNA replication genome-wide and describe how Rif1 opposes...