Biodentine Reduces Tumor Necrosis Factor Alpha–induced TRPA1 Expression in Odontoblastlike Cells

• Published in: Journal of endodontics Vol. 42; no. 4; pp. 589 - 595
• Main Authors: El Karim, Ikhlas A., BDS, PhD, McCrudden, Maelíosa T.C., BSc, PhD, McGahon, Mary K., BSc, PhD, Curtis, Tim M., BSc, PhD, Jeanneau, Charlotte, PhD, Giraud, Thomas, DDS, Irwin, Chris R., BSc, BDS, PhD, Linden, Gerard J., BSc, BDS, PhD, Lundy, Fionnuala T., BSc, PhD, About, Imad, MS, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2016; Elsevier
• Subjects: Biodentine; Calcium Channels - biosynthesis; Calcium Channels - genetics; Calcium Compounds - pharmacology; Cell Differentiation - drug effects; Dental Caries - metabolism; dental pulp; Dental Pulp - drug effects; Dental Pulp - pathology; Dental Pulp Capping; Dentistry; Endocrinology & Metabolism; Glycerophosphates - pharmacology; human; Humans; Immunohistochemistry; inflammation; Life Sciences; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - genetics; Odontoblasts - drug effects; Odontoblasts - metabolism; Odontoblasts - pathology; pulp capping; Silicates - pharmacology; transient receptor potential channel; Transient Receptor Potential Channels - biosynthesis; Transient Receptor Potential Channels - genetics; TRPA1 Cation Channel; TRPV Cation Channels - drug effects; TRPV Cation Channels - metabolism; Tumor Necrosis Factor-alpha - pharmacology; pulp capping; Biodentine; dental pulp; inflammation; human; transient receptor potential channel
• ISSN: 0099-2399; 1878-3554
• DOI: 10.1016/j.joen.2015.12.017

Abstract Introduction The transient receptor potential (TRP) ion channels have emerged as important cellular sensors in both neuronal and non-neuronal cells, with TRPA1 playing a central role in nociception and neurogenic inflammation. The functionality of TRP channels has been shown to be modulated by inflammatory cytokines. The aim of this study was to investigate the effect of inflammation on odontoblast TRPA1 expression and to determine the effect of Biodentine (Septodent, Paris, France) on inflammatory-induced TRPA1 expression. Methods Immunohistochemistry was used to study TRPA1 expression in pulp tissue from healthy and carious human teeth. Pulp cells were differentiated to odontoblastlike cells in the presence of 2 mmol/L beta-glycerophosphate, and these cells were used in quantitative polymerase chain reaction, Western blotting, calcium imaging, and patch clamp studies. Results Immunofluorescent staining revealed TRPA1 expression in odontoblast cell bodies and odontoblast processes, which was more intense in carious versus healthy teeth. TRPA1 gene expression was induced in cultured odontoblastlike cells by tumor necrosis factor alpha, and this expression was significantly reduced in the presence of Biodentine. The functionality of the TRPA1 channel was shown by calcium microfluorimetry and patch clamp recording, and our results showed a significant reduction in tumor necrosis factor alpha–induced TRPA1 responses after Biodentine treatment. Conclusions In conclusion, this study showed TRPA1 to be modulated by caries-induced inflammation and that Biodentine reduced TRPA1 expression and functional responses.