Analysis of mRNA decay and rRNA processing in Escherichia coli in the absence of RNase E‐based degradosome assembly
We demonstrate here that the assembly of the RNase E‐based degradosome of Escherichia coli is not required for normal mRNA decay in vivo. In contrast, deletion of the arginine‐rich RNA binding site (ARRBS) from the RNase E protein slightly impairs mRNA decay. When both the degradosome scaffold regio...
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Published in | Molecular microbiology Vol. 38; no. 4; pp. 854 - 866 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science, Ltd
01.11.2000
Blackwell Publishing Ltd |
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Abstract | We demonstrate here that the assembly of the RNase E‐based degradosome of Escherichia coli is not required for normal mRNA decay in vivo. In contrast, deletion of the arginine‐rich RNA binding site (ARRBS) from the RNase E protein slightly impairs mRNA decay. When both the degradosome scaffold region and the ARRBS are missing, mRNA decay is dramatically slowed, but 9S rRNA processing is almost normal. An extensive RNase E truncation mutation (rneδ610) had a more pronounced mRNA decay defect at 37°C than the temperature‐sensitive rne‐1 allele at 44°C. Taken together, these data suggest that the inviability associated with inactivation of RNase E is not related to defects in either mRNA decay or rRNA processing. |
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AbstractList | We demonstrate here that the assembly of the RNase E-based degradosome of Escherichia coli is not required for normal mRNA decay in vivo. In contrast, deletion of the arginine-rich RNA binding site (ARRBS) from the RNase E protein slightly impairs mRNA decay. When both the degradosome scaffold region and the ARRBS are missing, mRNA decay is dramatically slowed, but 9S rRNA processing is almost normal. An extensive RNase E truncation mutation (rnedelta610) had a more pronounced mRNA decay defect at 37 degrees C than the temperature-sensitive rne-1 allele at 44 degrees C. Taken together, these data suggest that the inviability associated with inactivation of RNase E is not related to defects in either mRNA decay or rRNA processing. We demonstrate here that the assembly of the RNase E-based degradosome of Escherichia coli is not required for normal mRNA decay in vivo. In contrast, deletion of the arginine-rich RNA binding site (ARRBS) from the RNase E protein slightly impairs mRNA decay. When both the degradosome scaffold region and the ARRBS are missing, mRNA decay is dramatically slowed, but 9S rRNA processing is almost normal. An extensive RNase E truncation mutation (rne delta 610) had a more pronounced mRNA decay defect at 37 degree C than the temperature-sensitive rne-1 allele at 44 degree C. Taken together, these data suggest that the inviability associated with inactivation of RNase E is not related to defects in either mRNA decay or rRNA processing. We demonstrate here that the assembly of the RNase E‐based degradosome of Escherichia coli is not required for normal mRNA decay in vivo . In contrast, deletion of the arginine‐rich RNA binding site (ARRBS) from the RNase E protein slightly impairs mRNA decay. When both the degradosome scaffold region and the ARRBS are missing, mRNA decay is dramatically slowed, but 9S rRNA processing is almost normal. An extensive RNase E truncation mutation ( rneδ610 ) had a more pronounced mRNA decay defect at 37°C than the temperature‐sensitive rne‐1 allele at 44°C. Taken together, these data suggest that the inviability associated with inactivation of RNase E is not related to defects in either mRNA decay or rRNA processing. We demonstrate here that the assembly of the RNase E‐based degradosome of Escherichia coli is not required for normal mRNA decay in vivo. In contrast, deletion of the arginine‐rich RNA binding site (ARRBS) from the RNase E protein slightly impairs mRNA decay. When both the degradosome scaffold region and the ARRBS are missing, mRNA decay is dramatically slowed, but 9S rRNA processing is almost normal. An extensive RNase E truncation mutation (rneδ610) had a more pronounced mRNA decay defect at 37°C than the temperature‐sensitive rne‐1 allele at 44°C. Taken together, these data suggest that the inviability associated with inactivation of RNase E is not related to defects in either mRNA decay or rRNA processing. |
Author | Kushner, Sidney R. Ow, Maria C. Liu, Qi |
Author_xml | – sequence: 1 givenname: Maria C. surname: Ow fullname: Ow, Maria C. – sequence: 2 givenname: Qi surname: Liu fullname: Liu, Qi – sequence: 3 givenname: Sidney R. surname: Kushner fullname: Kushner, Sidney R. |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/11115119$$D View this record in MEDLINE/PubMed |
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Snippet | We demonstrate here that the assembly of the RNase E‐based degradosome of Escherichia coli is not required for normal mRNA decay in vivo. In contrast, deletion... We demonstrate here that the assembly of the RNase E-based degradosome of Escherichia coli is not required for normal mRNA decay in vivo. In contrast, deletion... We demonstrate here that the assembly of the RNase E‐based degradosome of Escherichia coli is not required for normal mRNA decay in vivo . In contrast,... |
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SubjectTerms | degradosome degradosomes Endoribonucleases - genetics Endoribonucleases - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Regulation, Bacterial Multienzyme Complexes - genetics Multienzyme Complexes - metabolism Polyribonucleotide Nucleotidyltransferase - genetics Polyribonucleotide Nucleotidyltransferase - metabolism ribonuclease E RNA Helicases - genetics RNA Helicases - metabolism RNA, Bacterial - genetics RNA, Bacterial - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Ribosomal - genetics RNA, Ribosomal - metabolism rne gene rRNA |
Title | Analysis of mRNA decay and rRNA processing in Escherichia coli in the absence of RNase E‐based degradosome assembly |
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