Development of a high yielding E. coli periplasmic expression system for the production of humanized Fab' fragments - Ellis, Mark; Patel, Pareshkumar; Edon, Marjory; Ramage, Walter; Dickinson, Robert; Humphreys, David P. (Biotechnology progress, 2017)
Keywords: Antibodies, Monoclonal, Humanized - biosynthesis; Antibodies, Monoclonal, Humanized - chemistry; Antibodies, Monoclonal, Humanized - isolation & purification; DsbC; E coli; Escherichia coli; Escherichia coli - chemistry; Escherichia coli - genetics; Fab; Fermentation; Humans; Immunoglobulin Fab Fragments - biosynthesis; Immunoglobulin Fab Fragments - chemistry; Immunoglobulin Fab Fragments - isolation & purification; Mutation; Peptide Hydrolases - chemistry; Peptide Hydrolases - isolation & purification; Periplasm - chemistry; Periplasm - genetics; Protein Engineering; Protein Folding; Tsp; DsbC; Tsp; fermentation; Escherichia coli; Fab