Rational Design and Development of Near-Infrared-Emitting Firefly Luciferins Available In Vivo

• Published in: Angewandte Chemie International Edition Vol. 52; no. 4; pp. 1175 - 1179
• Main Authors: Kojima, Ryosuke, Takakura, Hideo, Ozawa, Takeaki, Tada, Yukio, Nagano, Tetsuo, Urano, Yasuteru
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 21.01.2013; WILEY‐VCH Verlag
• Subjects: Animals; Binding Sites; bioluminescence; Boron Compounds - chemistry; Carbocyanines - chemistry; Cell Line, Tumor; Fireflies - enzymology; Firefly Luciferin - chemistry; Firefly Luciferin - metabolism; Fluorescence Resonance Energy Transfer; Fluorescent Dyes - chemistry; HEK293 Cells; Humans; imaging agents; Luciferases, Firefly - chemistry; Luciferases, Firefly - metabolism; luciferin; Mice; Mice, Nude; Molecular Docking Simulation; near-infrared emission; Protein Structure, Tertiary; Quantum Dots; Research Design; Software; Spectroscopy, Near-Infrared; structure-activity relationships; Transplantation, Heterologous; Transplantation, Homologous

Shine on: A new rational design strategy for near‐infrared‐emitting firefly luciferins that are available in vivo has been developed using intramolecular bioluminescence resonance energy transfer (BRET). The emission wavelength could be freely tuned by the choice of BRET acceptor, and NIR biolumines...