Rational Design and Development of Near-Infrared-Emitting Firefly Luciferins Available In Vivo

Shine on: A new rational design strategy for near‐infrared‐emitting firefly luciferins that are available in vivo has been developed using intramolecular bioluminescence resonance energy transfer (BRET). The emission wavelength could be freely tuned by the choice of BRET acceptor, and NIR biolumines...

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Published inAngewandte Chemie International Edition Vol. 52; no. 4; pp. 1175 - 1179
Main Authors Kojima, Ryosuke, Takakura, Hideo, Ozawa, Takeaki, Tada, Yukio, Nagano, Tetsuo, Urano, Yasuteru
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 21.01.2013
WILEY‐VCH Verlag
Subjects
Animals
Binding Sites
bioluminescence
Boron Compounds - chemistry
Carbocyanines - chemistry
Cell Line, Tumor
Fireflies - enzymology
Firefly Luciferin - chemistry
Firefly Luciferin - metabolism
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
HEK293 Cells
Humans
imaging agents
Luciferases, Firefly - chemistry
Luciferases, Firefly - metabolism
luciferin
Mice
Mice, Nude
Molecular Docking Simulation
near-infrared emission
Protein Structure, Tertiary
Quantum Dots
Research Design
Software
Spectroscopy, Near-Infrared
structure-activity relationships
Transplantation, Heterologous
Transplantation, Homologous
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Summary:Shine on: A new rational design strategy for near‐infrared‐emitting firefly luciferins that are available in vivo has been developed using intramolecular bioluminescence resonance energy transfer (BRET). The emission wavelength could be freely tuned by the choice of BRET acceptor, and NIR bioluminescence could be detected in living cells and mice without the need for luciferase manipulation.
Bibliography:ArticleID:ANIE201205151
Ministry of Education, Culture, Sports, Science and Technology of Japan - No. 20117003; No. 23249004
ark:/67375/WNG-9WTK2KGL-L
JSPS - No. 22000006
This work was supported in part by a Grant-in-Aid for JSPS Fellows (to R.K.), by a Grant-in Aid for Specially Promoted Research (22000006, to T.N.), by a Basic Research Program from the Japan Science and Technology Agency (to Y.U.), and by research grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (20117003 and 23249004, to Y.U.). We thank Dr. Yoshihiro Hayakawa (The University of Tokyo) for providing Colon26-luc2 cells, and Ms. Naomi Misawa for helping us to construct the luciferase expression vectors.
Japan Science and Technology Agency
istex:C3D86C4B70FA6C8A04310A920EB32D6BAE86E6AA
This work was supported in part by a Grant‐in‐Aid for JSPS Fellows (to R.K.), by a Grant‐in Aid for Specially Promoted Research (22000006, to T.N.), by a Basic Research Program from the Japan Science and Technology Agency (to Y.U.), and by research grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (20117003 and 23249004, to Y.U.). We thank Dr. Yoshihiro Hayakawa (The University of Tokyo) for providing Colon26‐luc2 cells, and Ms. Naomi Misawa for helping us to construct the luciferase expression vectors.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201205151