Complexities associated with expression of Epstein-Barr virus (EBV) lytic origins of DNA replication

• Published in: Nucleic acids research Vol. 35; no. 10; pp. 3391 - 3406
• Main Authors: Xue, Shao-An, Griffin, Beverly E
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.05.2007; Oxford Publishing Limited (England)
• Subjects: Animals; Cell Line; DNA Replication; DNA, Viral - biosynthesis; Epstein-Barr virus; Gene Expression Regulation, Viral; Herpesvirus 4, Human - genetics; Herpesvirus 4, Human - metabolism; Herpesvirus 4, Human - physiology; Humans; Molecular Biology; Nuclease Protection Assays; Promoter Regions, Genetic; Replication Origin; Reverse Transcriptase Polymerase Chain Reaction; RNA, Antisense - analysis; RNA, Messenger - biosynthesis; RNA, Messenger - chemistry; RNA, Viral - biosynthesis; RNA, Viral - chemistry; Transcription Initiation Site; Transcription, Genetic; Viral Proteins - biosynthesis; Viral Proteins - genetics; Virus Replication

EBV has two lytic origins (oriLyt) of DNA replication lying at divergent sites on the viral genome within a duplicated sequence (DS). The latter contains potential hairpin loops, 'hinge' elements and the promoters for transcripts from viral genes BHLF1 and LF3. These genes themselves consi...