Gastrodia elata rhizoma ameliorates thioacetamide-induced liver injury in dogs

• Published in: Journal of advanced veterinary and animal research Vol. 10; no. 2; pp. 144 - 150
• Main Authors: Yoon, Hye-Bin, Cho, Jeong-Hwi, Kim, Jung-Soo, Kim, Jun-Hee, Oh, Hong-Geun, Kim, Chang-Su, Song, Young-Eun, Adam, Gareeballah Osman, Park, Yang-Gyu
• Format: Journal Article
• Language: English
• Published: Bangladesh Network for the Veterinarians of Bangladesh Bangladesh Agricultural Universityת Faculty of Veterinary Science 01.06.2023; A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET); Network for the Veterinarians of Bangladesh
• Subjects: Alanine; Alanine transaminase; Animals; Aquatic plants; Calibration; Chromatography; Dogs; Enzymes; Hematology; High performance liquid chromatography; Injury prevention; Liquid chromatography; Liver; Liver diseases; liver injury; gastrodia elata rhizome; nitric oxide; alt; dog; Mortality; Nitric oxide; Original; Oxidative stress; Parameters; Phosphates; Rhizomes; Silymarin; Solvents; Thioacetamide; Variance analysis; South Korea; ALT; Gastrodia elata rhizome; nitric oxide; dog; Liver injury
• ISSN: 2311-7710; 2311-7710
• DOI: 10.5455/javar.2023.j663

The study aimed to investigate the hepatoprotective effects of rhizome (GR) on thioacetamide (TAA)-induced liver injury in dogs. We evaluated serum biochemical and hematological parameters, with emphasis on alanine transaminase (ALT), alanine phosphates (ALP), and nitric oxide (NO) levels, in dogs with TAA-induced liver injury. The animals were divided into a control group (Con), TAA group, Silymarin group (Sil, 50 mg/kg), Gastrodia rhizome low dose (GRL) (low) + TAA, GRH (high) + TAA, and GR high-dose group (GRH) control group. GRL and GRH were given daily at 50 and 100 mg/kg, respectively. TAA was given on days 1, 4, and 7 at a dose of 300 mg/kg. GR significantly reduced liver injury in treated animals, as indicated by lowered levels of ALT (about 32% at day 21 in both GRL + TAA and GRH + TAA groups), ALP (about 17% and 21% at day 21 in both GRL + TAA, GRH + TAA groups, respectively), and NO (about 36% at day 21 in both GRL + TAA, GRH + TAA groups) compared to the TAA control group. Hematological parameters showed mild changes during the experiment. High-performance liquid chromatography analysis revealed gastrodin, a major component of the GR extract, constitutes 2.6% of the extract. The GR demonstrated significant hepatoprotective effects against TAA-induced liver injury in dogs. The study provides evidence for the potential therapeutic use of GR in the management of liver diseases.