Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients

• Published in: Biosensors & bioelectronics Vol. 169; p. 112643
• Main Authors: Steiner, Daniel J., Cognetti, John S., Luta, Ethan P., Klose, Alanna M., Bucukovski, Joseph, Bryan, Michael R., Schmuke, Jon J., Nguyen-Contant, Phuong, Sangster, Mark Y., Topham, David J., Miller, Benjamin L.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.12.2020
• Subjects: Antibodies, Viral - blood; antibody detection; Antigen microarray; antigens; Betacoronavirus - isolation & purification; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; biosensors; Coronavirus; Coronavirus - isolation & purification; Coronavirus Infections - blood; Coronavirus Infections - diagnosis; Coronavirus Infections - virology; COVID-19; COVID-19 infection; Equipment Design; HEK293 Cells; Humans; influenza; Influenza A virus - isolation & purification; Influenza virus; Influenza, Human - blood; Influenza, Human - diagnosis; Influenza, Human - virology; Label-free biosensor; microarray technology; Middle East Respiratory Syndrome Coronavirus - isolation & purification; monitoring; Pandemics; Pneumonia, Viral - blood; Pneumonia, Viral - diagnosis; Pneumonia, Viral - virology; Protein Array Analysis - instrumentation; Protein Array Analysis - methods; reflectometry; SARS Virus - isolation & purification; SARS-CoV-2; Sensitivity and Specificity; Severe acute respiratory syndrome coronavirus; Severe acute respiratory syndrome coronavirus 2; vaccine development; Antigen microarray; SARS-CoV-2; Coronavirus; Label-free biosensor; Influenza virus

Detection of antibodies to upper respiratory pathogens is critical to surveillance, assessment of the immune status of individuals, vaccine development, and basic biology. The urgent need for antibody detection tools has proven particularly acute in the COVID-19 era. We report a multiplex label-free...