Membrane protein isolation and structure determination in cell-derived membrane vesicles

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 120; no. 18; p. e2302325120
• Main Authors: Tao, Xiao, Zhao, Chen, MacKinnon, Roderick
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 02.05.2023
• Subjects: Binding Sites; Biological Sciences; Calcium ions; Calcium-binding protein; Cell Membrane - metabolism; Cell membranes; Cholesterol; Detergents; Integral membrane proteins; Ion channels; Ion Channels - metabolism; Lipids; Membrane Proteins - metabolism; Membrane vesicles; Membranes; Plasma membranes; Polymers; Protein structure; Proteins; Structural analysis; Vesicles; membrane protein; cell membrane vesicle; ion channel; cryo-EM; proteoliposome

Integral membrane protein structure determination traditionally requires extraction from cell membranes using detergents or polymers. Here, we describe the isolation and structure determination of proteins in membrane vesicles derived directly from cells. Structures of the ion channel Slo1 from tota...