Vibrio fortis sp. nov. and Vibrio hepatarius sp. nov., isolated from aquatic animals and the marine environment

• Published in: International journal of systematic and evolutionary microbiology Vol. 53; no. 5; pp. 1495 - 1501
• Main Authors: Thompson, F. L, Thompson, C. C, Hoste, B, Vandemeulebroecke, K, Gullian, M, Swings, J
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.09.2003; Society for General Microbiology
• Subjects: Animals; Bacteriology; Base Composition; Biological and medical sciences; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Marine; Marine Biology; Microbiology; Miscellaneous; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Systematics; Vibrio - classification; Vibrio - genetics; Vibrio - isolation & purification; Vibrio - metabolism; Vibrio fortis; Vibrio hepatarius; Aquatic environment; Bacteria; Vibrionaceae; Vibrio
• DOI: 10.1099/ijs.0.02658-0
• ISSN: 1466-5026

1 Laboratory for Microbiology, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium 2 BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium 3 National Center for Marine and Aquaculture Research, Guayaquil, Ecuador Correspondence F. L. Thompson Fabiano.Thompson{at}ugent.be In this study, the taxonomic positions of 19 Vibrio isolates disclosed in a previous study were evaluated. Phylogenetic analysis based on 16S rDNA sequences partitioned these isolates into groups that were closely related (98·8–99·1 % similarity) to Vibrio pelagius and Vibrio xuii , respectively. DNA–DNA hybridization experiments further showed that these groups had <70 % similarity to other Vibrio species. Two novel Vibrio species are proposed to accommodate these groups: Vibrio fortis sp. nov. (type strain, LMG 21557 T =CAIM 629 T ) and Vibrio hepatarius sp. nov. (type strain, LMG 20362 T =CAIM 693 T ). The DNA G+C content of both novel species is 45·6 mol%. Useful phenotypic features for discriminating V. fortis and V. hepatarius from other Vibrio species include production of indole and acetoin, utilization of cellobiose, fermentation of amygdalin, melibiose and mannitol, -galactosidase and tryptophan deaminase activities and fatty acid composition. Abbreviations: FAFLP, fluorescent amplified fragment length polymorphism; FAME, fatty acid methyl ester Published online ahead of print on 28 February 2003 as DOI 10.1099/ijs.0.02658-0. The GenBank/EMBL/DDBJ accession numbers for the 16S rDNA sequences of strains LMG 21557 T and LMG 20362 T are AJ514916 and AJ345063 , respectively. Tables of variable features among strains of V. fortis and V. hepatarius are available as supplementary material in IJSEM Online.