Temporal dynamics changes in the vaginal fluid microbiome: Implications for body fluid identification and estimating time since deposition (TsD) for forensics

• Published in: Forensic science international Vol. 364; p. 112219
• Main Authors: Ye, Linying, Liao, Lili, Lan, Jiangwei, Huang, Litao, Du, Jieyu, Zhang, Xiaofeng, Lun, Miaoqiang, Zhu, Bofeng, Liu, Chao, Chen, Ling
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.11.2024; Elsevier Limited
• Subjects: Adult; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Body fluid identification; Body fluids; Body Fluids - microbiology; chronic exposure; crime; Deposition; DNA, Bacterial; Error analysis; Exposure; feces; Feces - chemistry; Feces - microbiology; Female; Forensic medicine; Forensic science; Forensic Sciences; High-Throughput Nucleotide Sequencing; Humans; Indoor environments; Lactobacillus; Lactobacillus - isolation & purification; Male; Medical research; Microbial activity; Microbial changes; microbiome; Microbiomes; Microbiota; Microorganisms; Next-generation sequencing; Prediction models; RNA, Ribosomal, 16S; rRNA 16S; Saliva; Saliva - chemistry; Saliva - microbiology; Semen; Semen - microbiology; Sequence Analysis, DNA; Sex crimes; Sexual assault; Skin - microbiology; Taxonomy; temporal variation; Time dependence; Time Factors; Time series; Time since deposition (TsD); Vagina; Vagina - microbiology; Vaginal fluids; United States > US; China; Microbial changes; Exposure; Body fluid identification; Vaginal fluids; Time since deposition (TsD)

Vaginal fluid analysis plays a crucial role in sexual assault investigations. However, vaginal fluid found at crime scenes is usually subject to a certain duration of exposure. This study thus aimed to assess the influence of different durations of exposure to indoor environment on the vaginal micro...