Fluorescence microscopic visualization and quantification of initial bacterial colonization on enamel in situ

• Published in: Archives of oral biology Vol. 52; no. 11; pp. 1048 - 1056
• Main Authors: Hannig, C., Hannig, M., Rehmer, O., Braun, G., Hellwig, E., Al-Ahmad, A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2007
• Subjects: Adult; Advanced Basic Science; Animals; Bacteria - isolation & purification; Bacterial Adhesion; Biofilm; Biofilms; Cattle; Colony-Forming Units Assay; DAPI; Dental Enamel - microbiology; Dental Pellicle - microbiology; Dental Plaque - microbiology; Dentistry; Enamel; FISH; Humans; In situ; In Situ Hybridization, Fluorescence; Microbial Viability; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Pellicle; Plaque; Staining and Labeling; Time Factors; Enamel; In situ; FISH; Biofilm; Pellicle; DAPI; Plaque

The acquired salivary pellicle has been defined as proteinaceous film free of bacteria. However, due to the large numbers of microorganisms existent in the oral fluids, it is conceivable that adherent bacteria are already present in the initial pellicle. The aim of this in situ study was to visualiz...