Length oscillation induces force potentiation in infant guinea pig airway smooth muscle

• Published in: American journal of physiology. Lung cellular and molecular physiology Vol. 289; no. 6; pp. L909 - L915
• Main Authors: Wang, Lu, Chitano, Pasquale, Murphy, Thomas M
• Format: Journal Article
• Language: English
• Published: United States 01.12.2005
• Subjects: Actins - metabolism; Aging - physiology; Animals; Asthma - physiopathology; Bronchial Spasm - physiopathology; Constriction, Pathologic - physiopathology; Guinea Pigs; Humans; Muscle Contraction - physiology; Muscle, Smooth - growth & development; Respiration; Stress, Mechanical; Trachea - growth & development
• ISSN: 1040-0605; 1522-1504
• DOI: 10.1152/ajplung.00128.2005

Department of Pediatrics and Neonatal Perinatal Research Institute, Duke University Medical Center, Durham, North Carolina Submitted 22 March 2005 ; accepted in final form 20 May 2005 Deep inspiration counteracts bronchospasm in normal subjects but triggers further bronchoconstriction in hyperresponsive airways. Although the exact mechanisms for this contrary response by normal and hyperresponsive airways are unclear, it has been suggested that the phenomenon is related to changes in force-generating ability of airway smooth muscle after mechanical oscillation. It is known that healthy immature airways of both humans and animals exhibit hyperresponsiveness. We hypothesize that the profile of active force generation after mechanical oscillation changes with maturation and that this change contributes to the expression of airway hyperresponsiveness in juveniles. We examined the effect of an acute sinusoidal length oscillation on the force-generating ability of tracheal smooth muscle from 1 wk, 3 wk, and 2- to 3-mo-old guinea pigs. We found that the length oscillation produced 15–20% initial reduction in active force equally in all age groups. This was followed by a force recovery profile that displayed striking maturation-specific features. Unique to tracheal strips from 1-wk-old animals, active force potentiated beyond the maximal force generated before oscillation. We also found that actin polymerization was required in force recovery and that prostanoids contributed to the maturation-specific force potentiation in immature airway smooth muscle. Our results suggest a potentiated mechanosensitive contractile property of hyperresponsive airway smooth muscle. This can account for further bronchoconstriction triggered by deep inspiration in hyperresponsive airways. actin polymerization; deep inspiration; hyperresponsive airways; ontogenesis; prostanoids Address for reprint requests and other correspondence: L. Wang, Dept. of Pediatrics, Duke Univ. Medical Center, Rm. 302, Bell Bldg., Box 2994, Durham, NC 27710 (e-mail: lu.wang{at}duke.edu )