Fast separation of large biomolecules using short monolithic columns

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 927; pp. 80 - 89
• Main Authors: Podgornik, Aleš, Yamamoto, Shuichi, Peterka, Matjaž, Krajnc, Nika Lendero
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.05.2013
• Subjects: Analytics; antibodies; binding capacity; Binding sites; Biomolecues; Biomolecules; Chromatography; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; Convective Interaction Media (CIM); Macromolecules; Monoliths; Nucleic Acids - isolation & purification; Peak spreading; plasmids; Plasmids - isolation & purification; polynucleotides; Pressure drop; process monitoring; Proteins - isolation & purification; quality control; Ribonucleic acids; RNA; Separation; Short columns; Virion - isolation & purification; Viruses; Convective Interaction Media (CIM); Peak spreading; Monoliths; Biomolecues; Analytics; Short columns

► Application of short monolithic columns for analytics of macromolecules and nanoparticles – recent overview. ► Impact of macromolecule interactions on peak spreading and resolution in IEX. ► Overview of binding sites number for different class of macromolecules and nanoparticles. Chromatographic m...